Naturally fluorescent molecules may serve as cancer biomarker
Using rotational diffusion imaging, researchers can identify excess amounts of a naturally fluorescent molecule found in all living cells that could serve as a natural biomarker for cancer, according to a study published April 2 in the Journal of Photochemistry and Photobiology B: Biology.
Nicotinamide adenine dinucleotide (NADH) is a key coenzyme found mostly in the inner membrane of a cell's mitochondria. It fuels a series of biochemical reactions that involve various enzymes to produce ATP, the major energy source in cells. In the event of disease or a metabolic disorder, these enzymes and their related reactions can become disabled, causing a buildup of unused NADH.
"Dysfunctional enzymes in the mitochondria are known to be associated with serious health problems such as cancer and neurodegenerative diseases," said Ahmed Heikal, associate professor of bioengineering, Penn State. "By detecting the level of NADH and its distribution inside living cells, we should be able to monitor the mitochondrial activity and thus the integrity of any given cell, without adding potentially toxic dyes or actually destroying the cell."
Using a combination of spectroscopy and microscopy techniques, the researchers were able to convert such fluorescence into an accurate measure of NADH concentration in live cells, using it to tease apart the critical difference between normal and cancerous cells. Heikal and colleagues have found that the average concentration of NADH in breast cancer cells is about twice that in normal breast cells.
The researchers also looked at the amounts of NADH in the cell that is free and how much is bound to other enzymes. These amounts are different in normal and cancer cells.
"We realized that the fluorescence intensity not only depends upon the concentration of NADH but also on its structure -- free or enzyme-bound -- as well as its place inside the cell -- in the cytoplasm (non-nucleus part of the cell) or in mitochondria," explained Heikal. "Since a free NADH molecule would rotate -- tumble -- faster than enzyme-bound NADH, we were able to develop a technique called rotational diffusion imaging to establish a direct measure of the concentrations of free and enzyme-bound NADH throughout a living cell, whether in the cytosol [cell fluid] or the mitochondria."
To confirm their findings that disruption of chemical reactions that produce ATP can lead to an increase in NADH, the researchers exposed normal breast cells to potassium cyanide, a known inhibitor of some of these critical mitochondrial enzymes.
The researchers found that the NADH concentration in the normal cells increased when exposed to potassium cyanide. The relative amounts of NADH in the mitochondria also rose significantly.
Other researchers have previously measured the amount of NADH in cells using conventional biochemical techniques that require destroying the cells. However, Heikal believes measurements of dead cells provide no information about NADH distribution in the cells and may not be accurate or relevant for diagnostic or clinical use.
According to the results, the ability to accurately measure NADH levels in a cell without killing it could have potential implications for related research on human health and drug delivery.
"Our technique is not limited to detecting cancer. Other neurodegenerative diseases related to mitochondrial anomalies can also be detected with our method," Heikal said. "We can also use our approach to quantify the efficiency of a new drug on manipulating the activities of mitochondrial enzymes associated with energy production in cells."
The National Institutes of Health, National Science Foundation and Johnson & Johnson funded the work.
Nicotinamide adenine dinucleotide (NADH) is a key coenzyme found mostly in the inner membrane of a cell's mitochondria. It fuels a series of biochemical reactions that involve various enzymes to produce ATP, the major energy source in cells. In the event of disease or a metabolic disorder, these enzymes and their related reactions can become disabled, causing a buildup of unused NADH.
"Dysfunctional enzymes in the mitochondria are known to be associated with serious health problems such as cancer and neurodegenerative diseases," said Ahmed Heikal, associate professor of bioengineering, Penn State. "By detecting the level of NADH and its distribution inside living cells, we should be able to monitor the mitochondrial activity and thus the integrity of any given cell, without adding potentially toxic dyes or actually destroying the cell."
Using a combination of spectroscopy and microscopy techniques, the researchers were able to convert such fluorescence into an accurate measure of NADH concentration in live cells, using it to tease apart the critical difference between normal and cancerous cells. Heikal and colleagues have found that the average concentration of NADH in breast cancer cells is about twice that in normal breast cells.
The researchers also looked at the amounts of NADH in the cell that is free and how much is bound to other enzymes. These amounts are different in normal and cancer cells.
"We realized that the fluorescence intensity not only depends upon the concentration of NADH but also on its structure -- free or enzyme-bound -- as well as its place inside the cell -- in the cytoplasm (non-nucleus part of the cell) or in mitochondria," explained Heikal. "Since a free NADH molecule would rotate -- tumble -- faster than enzyme-bound NADH, we were able to develop a technique called rotational diffusion imaging to establish a direct measure of the concentrations of free and enzyme-bound NADH throughout a living cell, whether in the cytosol [cell fluid] or the mitochondria."
To confirm their findings that disruption of chemical reactions that produce ATP can lead to an increase in NADH, the researchers exposed normal breast cells to potassium cyanide, a known inhibitor of some of these critical mitochondrial enzymes.
The researchers found that the NADH concentration in the normal cells increased when exposed to potassium cyanide. The relative amounts of NADH in the mitochondria also rose significantly.
Other researchers have previously measured the amount of NADH in cells using conventional biochemical techniques that require destroying the cells. However, Heikal believes measurements of dead cells provide no information about NADH distribution in the cells and may not be accurate or relevant for diagnostic or clinical use.
According to the results, the ability to accurately measure NADH levels in a cell without killing it could have potential implications for related research on human health and drug delivery.
"Our technique is not limited to detecting cancer. Other neurodegenerative diseases related to mitochondrial anomalies can also be detected with our method," Heikal said. "We can also use our approach to quantify the efficiency of a new drug on manipulating the activities of mitochondrial enzymes associated with energy production in cells."
The National Institutes of Health, National Science Foundation and Johnson & Johnson funded the work.